Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter. Highlights • Thermostable—half life is more than 40 min at 95°C • Generates PCR products with 3'-dA overhangs • Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH 4) 2 SO 4. The latter allows for PCR at wide range of magnesium . Taq Polymerase. · Titanium® Taq PCR Kits User Manual PT www.doorway.ru PR0X Clontech Laboratories, Inc. A Takara Bio Company Page 6 of 11 5. T/A Cloning: Titanium Taq PCR products are compatible with T/A cloning methods. For best results, please observe the T/A cloning tips in Section V.B.
17 EP fermentas TrueStart™ Hot Start Taq DNA Polymerase 18 ER fermentas AluI 19 ER fermentas Alw21I (BsiHKAI) 20 ER fermentas Alw26I (BsmAI) 21 ER fermentas Alw44I 22 ER fermentas BamHI 23 ER fermentas BcnI (NciI) 24 ER fermentas BglI 25 ER fermentas BglII. primers. It includes DreamTaq DNA Polymerase, 2X DreamTaq buffer, dATP, dCTP, dGTP and dTTP, mM each, and 4 mM MgCl 2. DreamTaq buffer is a proprietary formulation optimized for robust performance in PCR. Rev d PROTOCOL 1. Gently vortex and briefly centrifuge DreamTaq PCR Master Mix (2X) after thawing. 2. Description The Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus www.doorway.ru enzyme catalyzes template-dependent polymerization of nucleotides into duplex DNA in the 5=3 direction. Taq DNA Polymerase has no detectable 3=5 exonuclease (proofreading) activity and possesses low 5=3 exonuclease activity.
Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter. Highlights • Thermostable—half life is more than 40 min at 95°C • Generates PCR products with 3'-dA overhangs • Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH 4) 2 SO 4. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming. Taq DNA Polymerase (recombinant), LC Component #EP #EP Taq DNA Polymerase, 1 U/µL U U 10X Taq Buffer with KCl mL2x 10X Taq Buffer with (NH 4) 2 SO 4 mL 2x mL 25 mM MgCl 2 mL 2x mL • Description Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. Thermo Scientific ™ Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl.
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